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Objective@#To investigate the influence of compound salvia injection on oxidative stress and complications in patients with gestational hypertension.@*Methods@#From August 2015 to Fabuary 2017, 140 patients with gestational hypertension in Binzhou Central Hospital were selected as study objects, and randomly divided into control group and research group according to the digital table, with 70 cases in each group.The control group was treated with routine treatment, the research group was given routine treatment combined with compound salvia injection.After treatment, SOD, MDA, NO, HMGB1, Cys C, hs-CRP, TNF-α, blood pressure and complications between the two groups were compared.@*Results@#After treatment, the levels of NO[(942.58±80.69)mmol/L vs.(734.51±78.63)mmol/L] and SOD[(124.65±23.81)U/mL vs.(90.64±24.13)U/mL] in the research group were higher than those in the control group, and the MAD level[(10.47±3.14)nmol/L vs.(15.82±3.32)nmol/L]in the research group was lower than that in the control group, the difference were statistically significant between the two groups(t=15.451, 8.394, 9.795, all P<0.05). After treatment, the levels of HMGB1[(1.81±0.43)mg/L vs.(3.89±0.65)mg/L], Cys C[(1.04±0.18)mg/L vs.(1.42±0.27)mg/L], TNF-α[(15.09±3.14)ng/L vs.(21.45±4.78)ng/L] and hs-CRP[(3.04±0.81)mg/L vs.(6.32±1.45)mg/L] in the research group were lower than those in the control group, the differences were statistically significant(t=22.329, 9.798, 9.304, 16.523, all P<0.05). After treatment, the systolic blood pressure[(131.25±5.74)mmHg vs.(139.61±5.59)mmHg] and diastolic blood pressure[(85.23±5.07)mmHg vs.(90.43±5.81)mmHg] in the research group were lower than those in the control group(t=8.730, 5.642, all P<0.05). The cesarean section rate(61.43% vs.87.14%) and intrauterine distress rate(1.43% vs.11.43%) in the research group were lower than those in the control group(χ2=12.115, 5.818, all P<0.05).@*Conclusion@#Compound salvia miltiorrhiza injection has significant control effect on oxidative stress and inflammatory reaction in patients with gestational hypertension, and is beneficial to reduce the complications of childbirth.
ABSTRACT
Objective To investigate the influence of compound salvia injection on oxidative stress and complications in patients with gestational hypertension.Methods From August 2015 to Fabuary 2017,140 patients with gestational hypertension in Binzhou Central Hospital were selected as study objects ,and randomly divided into control group and research group according to the digital table ,with 70 cases in each group.The control group was treated with routine treatment ,the research group was given routine treatment combined with compound salvia injec-tion.After treatment,SOD,MDA,NO,HMGB1,Cys C,hs-CRP,TNF-α,blood pressure and complications between the two groups were compared.Results After treatment,the levels of NO[(942.58 ±80.69)mmol／L vs.(734.51 ± 78.63)mmol／L] and SOD[(124.65 ±23.81)U／mL vs.(90.64 ±24.13)U／mL] in the research group were higher than those in the control group ,and the MAD level [(10.47 ±3.14) nmol／L vs.(15.82 ±3.32) nmol／L] in the research group was lower than that in the control group ,the difference were statistically significant between the two groups(t＝15.451,8.394,9.795,all P＜0.05).After treatment,the levels of HMGB1 [(1.81 ±0.43) mg／L vs. (3.89 ±0.65)mg／L],Cys C[(1.04 ±0.18)mg／L vs.(1.42 ±0.27) mg／L],TNF-α[(15.09 ±3.14) ng／L vs. (21.45 ±4.78) ng／L] and hs-CRP[(3.04 ±0.81) mg／L vs.(6.32 ±1.45) mg／L] in the research group were lower than those in the control group ,the differences were statistically significant (t＝22.329,9.798,9.304,16.523, all P＜0.05).After treatment,the systolic blood pressure[(131.25 ±5.74)mmHg vs.(139.61 ±5.59)mmHg] and diastolic blood pressure[(85.23 ±5.07)mmHg vs.(90.43 ±5.81) mmHg] in the research group were lower than those in the control group(t＝8.730,5.642,all P＜0.05).The cesarean section rate (61.43% vs.87.14%) and intrauterine distress rate(1.43%vs.11.43%) in the research group were lower than those in the control group (χ2 ＝12.115,5.818,all P＜0.05).Conclusion Compound salvia miltiorrhiza injection has significant control effect on oxidative stress and inflammatory reaction in patients with gestational hypertension ,and is beneficial to reduce the complications of childbirth.
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Objective To explore the effects of iPS cells-derived chimeric thymus transplantation on T cells reconstitution and graft versus host disease of murine after allo-BMT. Methods iPS cells-derived chimeric thymus was grafted under the renal capsules of mice after allogeneic IBM-BMT. The mice were divided into three groups:IBM-BMT group, IBM-BMT+TT group and IBM-BMT+DLI group. Four weeks after BMT, T lymphocyte subsets in the peripheral blood were analyzed by flow cytometry, the degree and pathological examination of GVHD were observed, respectively. Results Percentage of CD8+T cells in IBM-BMT group, IBM-BMT+TT group and IBM-BMT+DLI group was(5.52 ± 0.83)%,(11.10 ± 1.49)%and(8.49 ± 0.82)%respectively, there was signifi-cant difference between pairwise comparisons(P<0.05), and percentage of CD4 + T cells of the peripheral blood in IBM-BMT+TT group(9.60 ± 0.69)%was significantly higher than IBM-BMT group(6.42 ± 1.40)%and IBM-BMT+DLI group(8.07 ± 0.65)%(P<0.05) . IBM-BMT group and IBM-BMT+TT group showed less clinical and histopathological scoring of GVHD than IBM-BMT + DLI group. Conclusion iPS cells-derived chimeric thymus transplantation could effectively accelerate T cells reconstitution and prevent GVHD after allo-BMT.
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OBJECTIVE To assess the prevalence and analyze the influence factors of laryngopharyngeal reflux disease(LPRD) in the Fuzhou region, in order to provide a theoretical basis for the development of prevention and control measures for LPRD. METHODS A questionnaire survey in residents in Fuzhou by a random cluster sampling was carried out. Individual information, reflux symptom index(RSI) of Belafsky and risk factors were included. Patients more than 13 scores of RSI were defined as LPRD. Data were statistically analyzed. RESULTS A total of 4100 residents were investigated, 4063 of them were available. The prevalence of LPRD was 5.00%. Often eating too much, often drinking strong tea, menolipsis, rhinitis, tonsillitis were closely related to LPRD. CONCLUSION The prevalence of LPRD in Fuzhou region were closely related to many factors.
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Objective To examine an in vivo method for the differentiation of induced pluripotent stem cells (iPSCs) into thymic epithelial cells (TECs) in mice. Methods Green fluorescent protein-expressing iPS cells, derived from C57BL/6 mice, were injected into blastocysts from ICR mice. Chimeric blastocysts were then transferred into uteri of E2.5 pseudopregnant mice. Chimeric mouse could be identified by coat color 10 days after birth. The chimeric thymus was transplanted under the renal capsule of BALB/c nude mice. The spleen was cut out from the thymus-transplanted nude mice and the cells were dispersed and analyzed by a flow cytometer 4 weeks after transplantation. Results Chimeras were born 17 days after embryo transfer and 13 live-born chimeras were obtained. The contribution of iPSC-derived cells in the chimeras ranged from 5% to at most 90%. Typical thymic epithelium structure consisted of green fluorescent protein-expressing cells in chimera. The iPSCs-derived thymic epithelial cells could support the generation of new T cells. Conclusion The results indicate that mouse iPS cells can differentiate in vivo towards normally functioning TECs.